Light scattering measurements are commonly used to measure conformation-dependent properties of polymers, such as the hydrodynamic radius ( R h) and the radius of gyration ( R g), both of which are expected to change as a polypeptide chain undergoes collapse and adopts a secondary structure. Light scattering techniques have well-demonstrated applications in mechanistic investigations of protein folding. Balog, in Advanced Spectroscopic Methods to Study Biomolecular Structure and Dynamics, 2023 3.1 Protein folding and unfolding That said, for certain light scattering techniques such as DLS, MALS, and electrophoretic light scattering, sample preparation is extremely important and the material's and dispersant's optical properties must be known while other key physical properties of the sample must be controlled during the measurement.Įva Rose M. Critically, this information can be gained noninvasively, meaning sensitive or complex systems can be analyzed without unnecessary manipulation. These physical characteristics govern many of the properties of particles and their colloidal suspensions. ![]() When analyzed correctly, data from light scattering measurements can provide information on the average particle size, particle size distribution, polydispersity, molecular weight, and sometimes information on the particle shape. However, these theories rely on key assumptions, which must be understood in the context of the measurement and material being analyzed, otherwise the quality of the results will be questionable. Robust theories exist, which enable data to be analyzed and interpreted. For this reason a large number of methods and applications have been developed. ![]() Light scattering techniques allow rapid, often real-time, noninvasive analysis of many types of materials in environments ranging from manufacturing plants to field-based measurements from aircraft. Jonathan Moore, Eleonora Cerasoli, in Encyclopedia of Spectroscopy and Spectrometry (Third Edition), 2017 Conclusions
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